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Suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Candida tropicalis.

Chou HH, Chiou CS, Chen KW, Li SY

Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taipei 11561, Taiwan.

This study was designed to pursue the identification of optimal restriction enzyme for pulsed-field gel electrophoresis (PFGE) analysis of Candida tropicalis. Twelve restriction enzymes were tested on C. tropicalis strains. Our results indicate that BssHII, NaeI, and RsrII were useful enzymes for PFGE analysis, and NaeI was the best for PFGE analysis of C. tropicalis isolates.

Published 3 April 2007 in Diagn Microbiol Infect Dis, 57(4): 451-4.
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