Candida Research Today is a free monthly online journal that collates and summarizes the latest research about Candida, including details on thrush infections, yeast, diet, treatment, symptoms.

Immobilization of Candida rugosa lipase on a pH-sensitive support for enantioselective hydrolysis of ketoprofen ester.

Zhu S, Wu Y, Yu Z

School of Chemical Engineering and Pharmacy, Wuhan Institute of Chemical Technology, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan 430073, PR China. zhusd2003@21cn.com

Candida rugosa lipase (Lipase OF) was immobilized by covalent binding to a pH-sensitive support showing reversibly soluble-insoluble characteristics with pH change. The immobilized lipase could carry out the enantioselective hydrolysis of ketoprofen ester in a soluble form yet be recovered after precipitation by simply adjusting pH. Its activity and enantioselectivity for hydrolysis of 2-chloroethyl ester of ketoprofen were enhanced 1.5-fold and 8.7-fold compared with those of free lipase. After eight catalytic cycles, the immobilized enzyme was still 46% active and its enantioselectivity remained unchanged.

Published 7 March 2005 in J Biotechnol, 116(4): 397-401.
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