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Dynamic modification of microorganisms by pyrenebutanoate for fluorometric detection in capillary zone electrophoresis.

Horká M, Růzicka F, Holá V, Slais K

Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno. horka@iach.cz

Pyrenebutanoate, a fluorescent amphiphilic probe, is suggested here as a capillary zone electrophoresis (CZE) buffer additive for dynamic modification and analysis of microbial cells. Mixed cultures of microorganisms Escherichia coli, Candida albicans, Enterococcus faecalis and Staphylococcus epidermidis were concentrated, resolved by CZE and detected. Using UV excitation for on-column fluorometric detection, a detection sensitivity for the microorganisms on the order of from one to tens of injected cells was achieved.

Published 21 February 2005 in Electrophoresis, 26(3): 548-55.
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