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Use of denaturing high-performance liquid chromatography for rapid detection and identification of seven Candida species.

Goldenberg O, Herrmann S, Adam T, Marjoram G, Hong G, Göbel UB, Graf B

Institute of Microbiology and Hygiene, Charité Universitaetsmedizin Berlin, Campus Charité Mitte, Dorotheenstrasse 96, D-10117 Berlin, Germany.

A novel denaturing high-performance liquid chromatography (DHPLC)-based technique allows rapid high-resolution analysis of PCR products. We used this technique for unequivocal molecular identification of seven Candida species. We show the application of this PCR/DHPLC approach for direct detection and identification of yeast species from blood cultures and for detection of Candida colonization in the gastrointestinal tract of allogeneic transplant patients.

Published 7 December 2005 in J Clin Microbiol, 43(12): 5912-5.
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