Candida Research Today is a free monthly online journal that collates and summarizes the latest research about Candida, including details on thrush infections, yeast, diet, treatment, symptoms.

Role of oxidative phosphorylation in histatin 5-induced cell death in Saccharomyces cerevisiae.

De Smet K, Reekmans R, Contreras R

Unit of Fundamental and Applied Molecular Biology, Department for Molecular Biomedical Research, Ghent University and VIB, Technologiepark 927, B-9052 Ghent, Belgium.

The susceptibility of Saccharomyces cerevisiae to the anti-microbial peptide, histatin 5, was tested after pre-growth in fermentable and non-fermentable carbon sources and in the absence or presence of the uncoupler of oxidative phosphorylation, carbonyl cyanide m-chlorophenylhydrazone (CCCP). S. cerevisiae was more resistant to histatin 5 when grown on a fermentable carbon source compared to growth on a non-fermentable carbon source, indicating an important role for oxidative phosphorylation in histatin 5-induced cell death. Oxidative phosphorylation is a pre-requisite for histatin 5-induced cell death in Candida albicans but this is not the case in S. cerevisiae. Incubation of CCCP-treated S. cerevisiae cells with histatin 5 still resulted in cell death. These results suggest that histatin 5-induced cell death in S. cerevisiae differs from that in C. albicans.

Published 26 January 2005 in Biotechnol Lett, 26(23): 1781-5.
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