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Genotype distribution of Candida albicans isolates by 25S intron analysis with regard to invasiveness.

Karahan ZC, Güriz H, Ağirbaşli H, Balaban N, Göçmen JS, Aysev D, Akar N

Division of Pediatric Molecular Pathology and Genetics, Faculty of Medicine, Ankara University, Ankara, Turkey. ckarahan@medicine.ankara.edu.tr

The aim of this study was to genotype Candida albicans strains isolated from patients with invasive and non-invasive deep-seated infections. For this purpose, 301 C. albicans isolates (81 invasive and 220 non-invasive) were genotyped by using specific PCR primers designed to span the transposable group I intron of the 25S rDNA gene. Fifty-three of the 81 invasive isolates were genotype A (65.4%), eight were genotype B (9.9%) and 20 were genotype C (24.7%), while 98 of the 220 non-invasive isolates were genotype A (44.6%), 46 were genotype B (20.9%) and 76 were genotype C (34.5%). Genotype A was more prevalent among invasive isolates and genotypes B and C were more prevalent among non-invasive isolates (P = 0.0046). Genotypes D and E which represent C. dubliniensis were not found. These results indicate that there may be a relationship between C. albicans genotypes and invasiveness; genotype A being more invasive than others. The presence or absence of the transposable group I intron in the 25S rDNA gene may be important in determining the invasiveness of C. albicans.

Published 16 December 2004 in Mycoses, 47(11): 465-9.
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