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Transcriptional profiling of the early stages of germination in Candida albicans by real-time RT-PCR.

Toyoda M, Cho T, Kaminishi H, Sudoh M, Chibana H

Section of Infection Biology, Department of Functional Bioscience, Fukuoka Dental College, Fukuoka 814-0193, Japan.

By using real-time RT-PCR, we profiled the expression of CGR1, CaMSI3, EFG1, NRG1, and TUP1 in Candida albicans strains JCM9061 and CAI4 under several conditions, including induction of morphological transition, heat shock, and treatment with calcium inhibitors. Expression of CaMSI3 changed under these growth conditions except during heat shock. CGR1 expression increased during the early stages of hyphal growth in JCM9061, while expression was strain-dependent during heat shock. Both EFG1 and NRG1 were similarly expressed under hypha-inducing conditions and heat shock. Expression of TUP1 was slightly different from the expression of EFG1 or NRG1.

Published 23 November 2004 in FEMS Yeast Res, 5(3): 287-96.
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